Molecular classifiers for acute kidney transplant rejection in peripheral blood by whole genome gene expression profiling.

ABSTRACT: There are no minimally invasive diagnostic metrics for acute kidney transplant rejection (AR), especially in the setting of the common confounding diagnosis, acute dysfunction with no rejection (ADNR). Thus, though kidney transplant biopsies remain the gold standard, they are invasive, have substantial risks, sampling error issues and significant costs and are not suitable for serial monitoring. Global gene expression profiles of 148 peripheral blood samples from transplant patients with excellent function and normal histology (TX; n = 46), AR (n = 63) and ADNR (n = 39), from two independent cohorts were analyzed with DNA microarrays. We applied a new normalization tool, frozen robust multi-array analysis, particularly suitable for clinical diagnostics, multiple prediction tools to discove

SUBMITTER: Kurian SM 

PROVIDER: S-EPMC4439107 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature