A novel immunochromatographic test applied to a serological survey of Japanese encephalitis virus on pig farms in Korea.

ABSTRACT: Among vertebrate species, pigs are a major amplifying host of Japanese encephalitis virus (JEV) and measuring their seroconversion is a reliable indicator of virus activity. Traditionally, the hemagglutination inhibition test has been used for serological testing in pigs; however, it has several limitations and, thus, a more efficient and reliable replacement test is required. In this study, we developed a new immunochromatographic test for detecting antibodies to JEV in pig serum within 15 min. Specifically, the domain III region of the JEV envelope protein was successfully expressed in soluble form and used for developing the immunochromatographic test. The test was then applied to the surveillance of Japanese encephalitis (JE) in Korea. We found that our immunochromatographic test had g

SUBMITTER: Cha GW 

PROVIDER: S-EPMC4439121 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature