Bacteria in the injection water differently impacts the bacterial communities of production wells in high-temperature petroleum reservoirs.

ABSTRACT: Water flooding is widely used for oil recovery. However, how the introduction of bacteria via water flooding affects the subsurface ecosystem remains unknown. In the present study, the distinct bacterial communities of an injection well and six adjacent production wells were revealed using denaturing gradient gel electrophoresis (DGGE) and pyrosequencing. All sequences of the variable region 3 of the 16S rRNA gene retrieved from pyrosequencing were divided into 543 operational taxonomic units (OTUs) based on 97% similarity. Approximately 13.5% of the total sequences could not be assigned to any recognized phylum. The Unifrac distance analysis showed significant differences in the bacterial community structures between the production well and injection water samples. However, highly similar

SUBMITTER: Ren H 

PROVIDER: S-EPMC4439544 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature