Distribution of the type I interferon in different organs of chicken digestive system.

ABSTRACT:

Objective

Distribution of the type I interferon in different organs of the chicken digestive system.

Material and methods

In order to obtain a certain length (274 bp) of a fragment, a pair of primers was designed according to the conserved nucleotide sequence of gallus IFNAR-1 (EU477527.1) fragment that was published by the GenBank. The fragment was cloned by pEASY-T1 and amplified by relative fluorescence quantitative PCR with SYBR Green I; according to the results, we made a standard curve. The experimental group took interferon orally, while the control group took equivalent physiological saline orally, then we used relative fluorescence quantitative PCR to detect the copies of the IFNAR-1 gene of the palate, tongue, esophagus, craw, glandular stomach, duodenum and rectum

SUBMITTER: Guo SY 

PROVIDER: S-EPMC4439955 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature