Combined effect of tnf-α polymorphisms and hypoxia on steroid-induced osteonecrosis of femoral head.

ABSTRACT:

Objective

Tumor necrosis factor (TNF)-α is a proinflammatory cytokine, some studies reported that TNF-α gene plays important role in the pathogenesis of SONFH. And the polymorphisms of TNF-α were presented as risk factors for steroid-induced osteonecrosis of the femoral head (SONFH). Meanwhile, various environment factors involve in the pathogenesis of SONFH. Our study aimed to investigate the interaction effect of TNF-α polymorphisms and hypoxia factor on SONFH.

Methods

120 patients with SONFH and 100 healthy people, matched with the cases on age and sex, participated in this study. DNA was extracted from all participants. According to previous studies, genotyping of TNF-α polymorphisms (rs1800629, rs1799964 and rs1800630) was tested with the method of PCR-RDB (Reverse Dot

SUBMITTER: Liu Y 

PROVIDER: S-EPMC4440152 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature