Effects of Tris(1,3-dichloro-2-propyl) Phosphate (TDCPP) in Tetrahymena Thermophila: Targeting the Ribosome.

ABSTRACT: Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) has been frequently detected in the environment, and exposure to TDCPP appears widespread. It has been implicated to cause toxicity in vertebrates, but its potential to affect lower-trophic-level species remains unknown. In the present study, the ciliated protozoan, Tetrahymena thermophila, was used as a model to evaluate toxic effects of TDCPP and explore molecular mechanisms by integrating phenotypic observation, RNA-Seq and transmission electron microscopy (TEM) Imaging technologies. Exposure to 0.01, 0.1 or 1 μM TDCPP for 5 days significantly decreased the relative biomass by reducing number of cells, size of cells and quantity of cilia in a dose-dependent manner. RNA-Seq analysis demonstrated that expression of twenty-one ribosome protein

SUBMITTER: Li J 

PROVIDER: S-EPMC4440212 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature