Time-domain microfluidic fluorescence lifetime flow cytometry for high-throughput Forster resonance energy transfer screening.

ABSTRACT: Sensing ion or ligand concentrations, physico-chemical conditions, and molecular dimerization or conformation change is possible by assays involving fluorescent lifetime imaging. The inherent low throughput of imaging impedes rigorous statistical data analysis on large cell numbers. We address this limitation by developing a fluorescence lifetime-measuring flow cytometer for fast fluorescence lifetime quantification in living or fixed cell populations. The instrument combines a time-correlated single photon counting epifluorescent microscope with microfluidics cell-handling system. The associated computer software performs burst integrated fluorescence lifetime analysis to assign fluorescence lifetime, intensity, and burst duration to each passing cell. The maximum safe throughput of the i

SUBMITTER: Nedbal J 

PROVIDER: S-EPMC4440390 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature