Comparison of methods for the isolation of human breast epithelial and myoepithelial cells.

ABSTRACT: Two lineages, epithelial, and myoepithelial cells are the main cell populations in the normal mammary gland and in breast cancer. Traditionally, cancer research has been performed using commercial cell lines, but primary cell cultures obtained from fresh breast tissue are a powerful tool to study more reliably new aspects of mammary gland biology, including normal and pathological conditions. Nevertheless, the methods described to date have some technical problems in terms of cell viability and yield, which hamper work with primary mammary cells. Therefore, there is a need to optimize technology for the proper isolation of epithelial and myoepithelial cells. For this reason, we compared four methods in an effort to improve the isolation and primary cell culture of different cell population

SUBMITTER: Zubeldia-Plazaola A 

PROVIDER: S-EPMC4440402 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature