Detection of Peptide-based nanoparticles in blood plasma by ELISA.

ABSTRACT:

Aims

The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma.

Materials & methods

A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology.

Results

The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanop

SUBMITTER: Bode GH 

PROVIDER: S-EPMC4440766 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature