A customized light sheet microscope to measure spatio-temporal protein dynamics in small model organisms.

ABSTRACT: We describe a customizable and cost-effective light sheet microscopy (LSM) platform for rapid three-dimensional imaging of protein dynamics in small model organisms. The system is designed for high acquisition speeds and enables extended time-lapse in vivo experiments when using fluorescently labeled specimens. We demonstrate the capability of the setup to monitor gene expression and protein localization during ageing and upon starvation stress in longitudinal studies in individual or small groups of adult Caenorhabditis elegans nematodes. The system is equipped to readily perform fluorescence recovery after photobleaching (FRAP), which allows monitoring protein recovery and distribution under low photobleaching conditions. Our imaging platform is designed to easily switch between light sh

SUBMITTER: Rieckher M 

PROVIDER: S-EPMC4441442 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature