Synthetic and Evolutionary Construction of a Chlorate-Reducing Shewanella oneidensis MR-1.

ABSTRACT:

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Despite evidence for the prevalence of horizontal gene transfer of respiratory genes, little is known about how pathways functionally integrate within new hosts. One example of a mobile respiratory metabolism is bacterial chlorate reduction, which is frequently encoded on composite transposons. This implies that the essential components of the metabolism are encoded on these mobile elements. To test this, we heterologously expressed genes for chlorate reduction from Shewanella algae ACDC in the non-chlorate-reducing Shewanella oneidensis MR-1. The construct that ultimately endowed robust growth on chlorate included cld, a cytochrome c gene, clrABDC, and two genes of unknown function. Although strain MR-1 was unable to grow on chlorate after initial insertion of these gen

SUBMITTER: Clark IC 

PROVIDER: S-EPMC4442138 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature