Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis.

ABSTRACT:

Unlabelled

Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system. Previously, we described the major cohesin-dockerin interactions of the cellulosome components, and on this basis a model of diverse cellulosome assemblies was derived. In this work, we cultivated C. clariflavum on cellobiose-, microcrystalline cellulose-, and switchgrass-containing media and isolated cell-free cellulosome complexes from each culture. Gel filtration separation of the cellulosome samples revealed two major fractions, which were analyzed by label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) in order to identify the key players of the cellulosome assemblies there

SUBMITTER: Artzi L 

PROVIDER: S-EPMC4442141 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature