Cotranslational coat protein-mediated inhibition of potyviral RNA translation.

ABSTRACT:

Unlabelled

Potato virus A (PVA) is a single-stranded positive-sense RNA virus and a member of the family Potyviridae. The PVA coat protein (CP) has an intrinsic capacity to self-assemble into filamentous virus-like particles, but the mechanism responsible for the initiation of viral RNA encapsidation in vivo remains unclear. Apart from virion assembly, PVA CP is also involved in the inhibition of viral RNA translation. In this study, we show that CP inhibits PVA RNA translation in a dose-dependent manner, through a mechanism involving the CP-encoding region. Analysis of this region, however, failed to identify any RNA secondary structure(s) preferentially recognized by CP, suggesting that the inhibition depends on CP-CP rather than CP-RNA interactions. In agreement with this possib

SUBMITTER: Besong-Ndika J 

PROVIDER: S-EPMC4442359 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature