Phosphorylation controls the nuclear-cytoplasmic shuttling of influenza A virus nucleoprotein.

ABSTRACT:

Unlabelled

The nucleoprotein (NP) is a major component of the viral ribonucleoprotein (vRNP) complex. During the replication of influenza virus, the vRNP complex undergoes nuclear-cytoplasmic shuttling, during which NP serves as one of the determinants. To date, many phosphorylation sites on NP have been identified, but the biological functions of many of these phosphorylation sites remain unknown. In the present study, the functions of the phosphorylation sites S9, Y10, and Y296 were characterized. These residues are highly conserved, and their phosphorylation was essential for virus growth in cell culture and in a mouse model by regulating the activity of the viral polymerase and the nuclear-cytoplasmic shuttling of NP. The phosphorylation and dephosphorylation of S9 and Y10 cont

SUBMITTER: Zheng W 

PROVIDER: S-EPMC4442427 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature