Effect of Ipr1 on expression levels of immune genes related to macrophage anti-infection of mycobacterium tuberculosis.

ABSTRACT:

Background

Intracellular pathogen resistance 1 (Ipr1) has been found in macrophages and plays a pivotal role in fighting against Mycobacterium tuberculosis (Mtb) infection. This study is designed to evaluate the effect of Iprl on the expression of macrophage genes related to the anti-infection of Mtb. Design or methods: In the experimental and control groups, the macrophages were infected with Mycobacterium H37Ra, and then the related immune genes between two groups were detected using microarray assay. Real-time quantitative PCR was applied to detect the differences in the expression of three up-regulated genes detected by microarray assay and to verify the reliability of microarray assay.

Results

The expression of Iprl up-regulated 11 genes related to macrophage anti-immun

SUBMITTER: Li N 

PROVIDER: S-EPMC4443065 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature