Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro.

ABSTRACT: Human cell reprogramming technologies offer access to live human neurons from patients and provide a new alternative for modeling neurological disorders in vitro. Neural electrical activity is the essence of nervous system function in vivo. Therefore, we examined neuronal activity in media widely used to culture neurons. We found that classic basal media, as well as serum, impair action potential generation and synaptic communication. To overcome this problem, we designed a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concentrations of inorganic salts, neuroactive amino acids, and energetic substrates. We then tested that this medium adequately supports neuronal activity and survival of human neurons in culture. Long-term exposure to this physiolo

SUBMITTER: Bardy C 

PROVIDER: S-EPMC4443325 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature