Identification of novel long noncoding RNAs underlying vertebrate cardiovascular development.

ABSTRACT:

Background

Long noncoding RNAs (lncRNAs) have emerged as critical epigenetic regulators with important functions in development and disease. Here, we sought to identify and functionally characterize novel lncRNAs critical for vertebrate development.

Methods and results

By relying on human pluripotent stem cell differentiation models, we investigated lncRNAs differentially regulated at key steps during human cardiovascular development with a special focus on vascular endothelial cells. RNA sequencing led to the generation of large data sets that serve as a gene expression roadmap highlighting gene expression changes during human pluripotent cell differentiation. Stage-specific analyses led to the identification of 3 previously uncharacterized lncRNAs, TERMINATOR, ALIEN, and P

SUBMITTER: Kurian L 

PROVIDER: S-EPMC4443804 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature