Tuning scaffold mechanics by laminating native extracellular matrix membranes and effects on early cellular remodeling.

ABSTRACT: At approximately 50 µm thin, the human amniotic membrane (hAM) has been shown to be a versatile biomaterial with applications ranging from ocular transplants to skin and nerve regeneration. These investigations describe laminating layers of the hAM into a multilayered, conformation creating a thicker, more robust biomaterial for applications requiring more supportive structures. Amniotic membranes were decellularized using 4 M NaCl and prepared as either flat single-layered sheets or rolled into concentric five-layered configurations. Constructs were seeded with human vascular smooth muscle cells and cultured over 40 days to quantify biological and mechanical changes that occurred during early remodeling events. By day 40 single-layered constructs displayed a decreasing trend in cellular d

SUBMITTER: Amensag S 

PROVIDER: S-EPMC4443916 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature