3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates

ABSTRACT: The histological assessment of spinal cord tissue in three dimensions has previously been very time consuming and prone to errors of interpretation. Advances in tissue clearing have significantly improved visualization of fluorescently labelled axons. While recent proof-of-concept studies have been performed with transgenic mice in which axons were prelabeled with GFP, investigating axonal regeneration requires stringent axonal tracing methods as well as the use of animal models in which transgenic axonal labeling is not available. Using rodent models of spinal cord injury, we labeled axon tracts of interest using both adeno-associated virus and chemical tracers and performed tetrahydrofuran-based tissue clearing to image multiple axon types in spinal cords using light sheet and confocal m

SUBMITTER: Soderblom C 

PROVIDER: S-EPMC4444235 | biostudies-literature | 2015 Mar-Apr

REPOSITORIES: biostudies-literature