Three-dimensional, soft neotissue arrays as high throughput platforms for the interrogation of engineered tissue environments.

ABSTRACT: Local signals from tissue-specific extracellular matrix (ECM) microenvironments, including matrix adhesive ligand, mechanical elasticity and micro-scale geometry, are known to instruct a variety of stem cell differentiation processes. Likewise, these signals converge to provide multifaceted, mechanochemical cues for highly-specific tissue morphogenesis or regeneration. Despite accumulated knowledge about the individual and combined roles of various mechanochemical ECM signals in stem cell activities on 2-dimensional matrices, the understandings of morphogenetic or regenerative 3-dimenstional tissue microenvironments remain very limited. To that end, we established high-throughput platforms based on soft, fibrous matrices with various combinatorial ECM proteins meanwhile highly-tunable in e

SUBMITTER: Floren M 

PROVIDER: S-EPMC4444363 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature