Alpha-L-fucosidase isoenzyme iso2 from Paenibacillus thiaminolyticus.

ABSTRACT:

Background

α-L-Fucosidases are enzymes involved in metabolism of α-L-fucosylated molecules, compounds with a fundamental role in different life essential processes including immune response, fertilization and development, but also in some serious pathological events. According to the CAZy database, these enzymes belong to families 29 and 95. Some of them are also reported to be able to catalyze transglycosylation reactions, during which α-L-fucosylated molecules, representing compounds of interest especially for pharmaceutical industry, are formed.

Methods

Activity-based screening of a genomic library was used to isolate the gene encoding a novel α-L-fucosidase. The enzyme was expressed in E.coli and affinity chromatography was used for purification of His-tagged α-L-fucosid

SUBMITTER: Benesova E 

PROVIDER: S-EPMC4445282 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature