Functional bottlenecks for generation of HIV-1 intersubtype Env recombinants.

ABSTRACT:

Background

Intersubtype recombination is a powerful driving force for HIV evolution, impacting both HIV-1 diversity within an infected individual and within the global epidemic. This study examines if viral protein function/fitness is the major constraint shaping selection of recombination hotspots in replication-competent HIV-1 progeny. A better understanding of the interplay between viral protein structure-function and recombination may provide insights into both vaccine design and drug development.

Results

In vitro HIV-1 dual infections were used to recombine subtypes A and D isolates and examine breakpoints in the Env glycoproteins. The entire env genes of 21 A/D recombinants with breakpoints in gp120 were non-functional when cloned into the laboratory strain, NL4-3. Lik

SUBMITTER: Bagaya BS 

PROVIDER: S-EPMC4445978 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature