Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells.

ABSTRACT:

Background

Indoleamine 2,3-dioxygenase (IDO) is an enzyme associated with the regulation of immune responses. Cytokines such as IFNγ induce its expression in endothelial cells originating from immune-privileged sites. In this study, we investigate regulators of IDO in primary endothelial cells from a non-immune-privileged site and determine whether IDO expression affects immune cell behavior.

Methods

IDO expression was determined using real-time quantitative polymerase chain reaction and immunoblotting. IDO activity was estimated using an IDO enzyme assay. Primary cells were transfected using microporation, and T-cell migration was determined using a cell transmigration assay.

Results

IDO is expressed in human saphenous vein endothelial cells after stimulation with IF

SUBMITTER: Mouratidis PX 

PROVIDER: S-EPMC4446016 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature