Characterization and clinical evaluation of CD10+ stroma cells in the breast cancer microenvironment.

ABSTRACT:

Purpose

There is growing evidence that interaction between stromal and tumor cells is pivotal in breast cancer progression and response to therapy. Based on earlier research suggesting that during breast cancer progression, striking changes occur in CD10(+) stromal cells, we aimed to better characterize this cell population and its clinical relevance.

Experimental design

We developed a CD10(+) stroma gene expression signature (using HG U133 Plus 2.0) on the basis of the comparison of CD10 cells isolated from tumoral (n = 28) and normal (n = 3) breast tissue. We further characterized the CD10(+) cells by coculture experiments of representative breast cancer cell lines with the different CD10(+) stromal cell types (fibroblasts, myoepithelial, and mesenchymal stem cells). We th

SUBMITTER: Desmedt C 

PROVIDER: S-EPMC4446057 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature