Selective recruitment of nuclear factors to productively replicating herpes simplex virus genomes.

ABSTRACT: Much of the HSV-1 life cycle is carried out in the cell nucleus, including the expression, replication, repair, and packaging of viral genomes. Viral proteins, as well as cellular factors, play essential roles in these processes. Isolation of proteins on nascent DNA (iPOND) was developed to label and purify cellular replication forks. We adapted aspects of this method to label viral genomes to both image, and purify replicating HSV-1 genomes for the identification of associated proteins. Many viral and cellular factors were enriched on viral genomes, including factors that mediate DNA replication, repair, chromatin remodeling, transcription, and RNA processing. As infection proceeded, packaging and structural components were enriched to a greater extent. Among the more abundant proteins th

SUBMITTER: Dembowski JA 

PROVIDER: S-EPMC4446364 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature