Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.

ABSTRACT: Organisms have different circuitries that allow converting signal molecule levels to changes in gene expression. An important challenge in synthetic biology involves the de novo design of RNA modules enabling dynamic signal processing in live cells. This requires a scalable methodology for sensing, transmission, and actuation, which could be assembled into larger signaling networks. Here, we present a biochemical strategy to design RNA-mediated signal transduction cascades able to sense small molecules and small RNAs. We design switchable functional RNA domains by using strand-displacement techniques. We experimentally characterize the molecular mechanism underlying our synthetic RNA signaling cascades, show the ability to regulate gene expression with transduced RNA signals, and describe

SUBMITTER: Shen S 

PROVIDER: S-EPMC4446421 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature