Single-Round Patterned DNA Library Microarray Aptamer Lead Identification.

ABSTRACT: A method for identifying an aptamer in a single round was developed using custom DNA microarrays containing computationally derived patterned libraries incorporating no information on the sequences of previously reported thrombin binding aptamers. The DNA library was specifically designed to increase the probability of binding by enhancing structural complexity in a sequence-space confined environment, much like generating lead compounds in a combinatorial drug screening library. The sequence demonstrating the highest fluorescence intensity upon target addition was confirmed to bind the target molecule thrombin with specificity by surface plasmon resonance, and a novel imino proton NMR/2D NOESY combination was used to screen the structure for G-quartet formation. We propose that the lack o

SUBMITTER: Martin JA 

PROVIDER: S-EPMC4446497 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature