Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues.

ABSTRACT: Protein and transcript levels are partly decoupled as a function of translation efficiency and protein degradation. Selective protein degradation via the Ubiquitin-26S proteasome system (UPS) ensures protein homeostasis and facilitates adjustment of protein abundance during changing environmental conditions. Since individual leaf tissues have specialized functions, their protein composition is different and hence also protein level regulation is expected to differ. To understand UPS function in a tissue-specific context we developed a method termed Meselect to effectively and rapidly separate Arabidopsis thaliana leaf epidermal, vascular and mesophyll tissues. Epidermal and vascular tissue cells are separated mechanically, while mesophyll cells are obtained after rapid protoplasting. The h

SUBMITTER: Svozil J 

PROVIDER: S-EPMC4446536 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature