IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2.

ABSTRACT:

Purpose

Cockroaches are the second leading allergen in Taiwan. Sensitization to Per a 2, the major American cockroach allergen, correlates with clinical severity among patients with airway allergy, but there is limited information on IgE epitopes and tissue localization of Per a 2. This study aimed to identify Per a 2 linear IgE-binding epitopes and its distribution in the body of a cockroach.

Methods

The cDNA of Per a 2 was used as a template and combined with oligonucleotide primers specific to the target areas with appropriate restriction enzyme sites. Eleven overlapping fragments of Per a 2 covering the whole allergen molecule, except 20 residues of signal peptide, were generated by PCR. Mature Per a 2 and overlapping deletion mutants were affinity-purified and assayed f

SUBMITTER: Lee MF 

PROVIDER: S-EPMC4446636 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature