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Promoter-Autonomous Functioning in a Controlled Environment using Single Molecule FISH.

ABSTRACT: Transcription is a highly regulated biological process, initiated through the assembly of complexes at the promoter that contain both the general transcriptional machinery and promoter-specific factors. Despite the abundance of studies focusing on transcription, certain questions have remained unanswered. It is not clear how the transcriptional profile of a promoter is affected by genomic context. Also, there is no single cell method to directly compare transcriptional profiles independent of gene length and sequence. In this work, we employ a single genetic site for isolating the transcriptional kinetics of yeast promoters. Utilizing single molecule FISH, we directly compare the transcriptional activity of different promoters, considering both synthesis and cell-to-cell variability. With this approach, we provide evidence suggesting promoters autonomously encode their associated transcriptional profiles, independent of genomic locus, gene length and gene sequence.

SUBMITTER: Hocine S

PROVIDER: S-EPMC4446897 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Promoter-Autonomous Functioning in a Controlled Environment using Single Molecule FISH.

Hocine Sami S Vera Maria M Zenklusen Daniel D Singer Robert H RH

Scientific reports 20150528

Transcription is a highly regulated biological process, initiated through the assembly of complexes at the promoter that contain both the general transcriptional machinery and promoter-specific factors. Despite the abundance of studies focusing on transcription, certain questions have remained unanswered. It is not clear how the transcriptional profile of a promoter is affected by genomic context. Also, there is no single cell method to directly compare transcriptional profiles independent of ge ...[more]

PMID: 26017315

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