Effects of partial silencing of genes coding for enzymes involved in glycolysis and tricarboxylic acid cycle on the enterance of human fibroblasts to the S phase.

ABSTRACT:

Background

Previously published reports indicated that some enzymes of the central carbon metabolism (CCM), particularly those involved in glycolysis and the tricarboxylic acid cycle, may contribute to regulation of DNA replication. However, vast majority of such works was performed with the use of cancer cells, in the light of carcinogenesis. On the other hand, recent experiments conducted on bacterial models provided evidence for the direct genetic link between CCM and DNA replication. Therefore, we asked if silencing of genes coding for glycolytic and/or Krebs cycle enzymes may affect the control of DNA replication in normal human fibroblasts.

Results

Particular genes coding for these enzymes were partially silenced with specific siRNAs. Such cells remained viable. We fou

SUBMITTER: Konieczna A 

PROVIDER: S-EPMC4446904 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature