Temporal hierarchy of gene expression mediated by transcription factor binding affinity and activation dynamics.

ABSTRACT:

Unlabelled

Understanding cellular responses to environmental stimuli requires not only the knowledge of specific regulatory components but also the quantitative characterization of the magnitude and timing of regulatory events. The two-component system is one of the major prokaryotic signaling schemes and is the focus of extensive interest in quantitative modeling and investigation of signaling dynamics. Here we report how the binding affinity of the PhoB two-component response regulator (RR) to target promoters impacts the level and timing of expression of PhoB-regulated genes. Information content has often been used to assess the degree of conservation for transcription factor (TF)-binding sites. We show that increasing the information content of PhoB-binding sites in designed ph

SUBMITTER: Gao R 

PROVIDER: S-EPMC4447250 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature