Natural atypical Listeria innocua strains with Listeria monocytogenes pathogenicity island 1 genes.

ABSTRACT: Identification of bona fide Listeria isolates into the six species of the genus normally requires only a few tests. Aberrant isolates do occur, but even then only one or two extra confirmatory tests are generally needed for identification to species level. We have discovered a hemolytic-positive, rhamnose and xylose fermentation-negative Listeria strain with surprising recalcitrance to identification to the species level due to contradictory results in standard confirmatory tests. The issue had to be resolved by using total DNA-DNA hybridization testing and then confirmed by further specific PCR-based tests including a Listeria microarray assay. The results show that this isolate is indeed a novel one. Its discovery provides the first fully documented instance of a hemolytic Listeria innoc

SUBMITTER: Johnson J 

PROVIDER: S-EPMC444784 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature