Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB.

ABSTRACT:

Background

The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the Pi starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ΔphoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR.

Results

DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to pstS promoter DNA in vitro. Gel mobility shift assays and mutational analysis of the pstS promoter region revealed that RamB binds to two sites localized at positions -74 to -88 and -9 to +2 with respect to the transcriptional

SUBMITTER: Sorger-Herrmann U 

PROVIDER: S-EPMC4448153 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature