In vitro comparative study of two decellularization protocols in search of an optimal myocardial scaffold for recellularization.

ABSTRACT: INTRODUCTION:Selection of a biomaterial-based scaffold that mimics native myocardial extracellular matrix (ECM) architecture can facilitate functional cell attachment and differentiation. Although decellularized myocardial ECM accomplishes these premises, decellularization processes may variably distort or degrade ECM structure. MATERIALS AND METHODS:Two decellularization protocols (DP) were tested on porcine heart samples (epicardium, mid myocardium and endocardium). One protocol, DP1, was detergent-based (SDS and Triton X-100), followed by DNase I treatment. The other protocol, DP2, was focused in trypsin and acid with Triton X-100 treatments. Decellularized myocardial scaffolds were reseeded by embedding them in RAD16-I peptidic hydrogel with adipose tissue-derived progenitor cells (ATD

SUBMITTER: Perea-Gil I 

PROVIDER: S-EPMC4448195 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature