Temporal and spatial profiles of chitinase expression by norway spruce in response to bark colonization by Heterobasidion annosum.

ABSTRACT: Pathogen colonization and transcript levels of three host chitinases, putatively representing classes I, II, and IV, were monitored with real-time PCR after wounding and bark infection by Heterobasidion annosum in 32-year-old trees of Norway spruce (Picea abies) with low (clone 409) or high (clone 589) resistance to this pathogen. Three days after inoculation, comparable colonization levels were observed in both clones in the area immediately adjacent to inoculation. At 14 days after infection, pathogen colonization was restricted to the area immediately adjacent to the site of inoculation for clone 589 but had progressed further into the host tissue in clone 409. Transcript levels of the class II and IV chitinases increased after wounding or inoculation, but the transcript level of the cl

SUBMITTER: Hietala AM 

PROVIDER: S-EPMC444825 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature