Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes.

ABSTRACT: Transcription factors are abundant Sumo targets, yet the global distribution of Sumo along the chromatin and its physiological relevance in transcription are poorly understood. Using Saccharomyces cerevisiae, we determined the genome-wide localization of Sumo along the chromatin. We discovered that Sumo-enriched genes are almost exclusively involved in translation, such as tRNA genes and ribosomal protein genes (RPGs). Genome-wide expression analysis showed that Sumo positively regulates their transcription. We also discovered that the Sumo consensus motif at RPG promoters is identical to the DNA binding motif of the transcription factor Rap1. We demonstrate that Rap1 is a molecular target of Sumo and that sumoylation of Rap1 is important for cell viability. Furthermore, Rap1 sumoylation p

SUBMITTER: Chymkowitch P 

PROVIDER: S-EPMC4448685 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature