Choice of antiretroviral therapy differentially impacts survival of HIV-infected CD4 T cells.

ABSTRACT:

Background

HIV eradication strategies are now being evaluated in vitro and in vivo. A cornerstone of such approaches is maximal suppression of viral replication with combination antiretroviral therapy (ART). Since many antiretroviral agents have off target effects, and different classes target different components of the viral life cycle, we questioned whether different classes of ART might differentially affect the survival and persistence of productively HIV-infected CD4 T cells.

Methods

In vitro infections of primary CD4 T cells using clinical isolates of HIV-1 that were either protease inhibitor susceptible (HIV PI-S), or resistant (HIV PI-R) were treated with nothing, lopinavir, efavirenz or raltegravir. Cell viability, apoptosis, and the proportion of surviving cells t

SUBMITTER: Cummins NW 

PROVIDER: S-EPMC4448955 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature