Bioengineering a human plasma-based epidermal substitute with efficient grafting capacity and high content in clonogenic cells.

ABSTRACT:

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Cultured epithelial autografts (CEAs) produced from a small, healthy skin biopsy represent a lifesaving surgical technique in cases of full-thickness skin burn covering >50% of total body surface area. CEAs also present numerous drawbacks, among them the use of animal proteins and cells, the high fragility of keratinocyte sheets, and the immaturity of the dermal-epidermal junction, leading to heavy cosmetic and functional sequelae. To overcome these weaknesses, we developed a human plasma-based epidermal substitute (hPBES) for epidermal coverage in cases of massive burn, as an alternative to traditional CEA, and set up critical quality controls for preclinical and clinical studies. In this study, phenotypical analyses in conjunction with functional assays (clonal analysi

SUBMITTER: Alexaline MM 

PROVIDER: S-EPMC4449090 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature