Yes-associated protein (YAP) is a negative regulator of chondrogenesis in mesenchymal stem cells.

ABSTRACT:

Introduction

The control of differentiation of mesenchymal stromal/stem cells (MSCs) is crucial for tissue engineering strategies employing MSCs. The purpose of this study was to investigate whether the transcriptional co-factor Yes-associated protein (YAP) regulates chondrogenic differentiation of MSCs.

Methods

Expression of total YAP, its paralogue transcriptional co-activator with PDZ-binding motif (TAZ), and individual YAP transcript variants during in vitro chondrogenesis of human MSCs was determined by quantitative reverse transcription polymerase chain reaction (RT-PCR). YAP expression was confirmed by western blotting. To determine the effect of high YAP activity on chondrogenesis, C3H10T1/2 MSC-like cells were transduced with human (h)YAP and treated in micromass wi

SUBMITTER: Karystinou A 

PROVIDER: S-EPMC4449558 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature