Controlled Endolysosomal Release of Agents by pH-responsive Polymer Blend Particles.

ABSTRACT:

Purpose

A key step of delivering extracellular agents to its intracellular target is to escape from endosomal/lysosomal compartments, while minimizing the release of digestive enzymes that may compromise cellular functions. In this study, we examined the intracellular distribution of both fluorecent cargoes and enzymes by a particle delivery platform made from the controlled blending of poly(lactic-co-glycolic acid) (PLGA) and a random pH-sensitive copolymer.

Methods

We utilized both microscopic and biochemical methods to semi-quantitatively assess how the composition of blend particles affects the level of endosomal escape of cargos of various sizes and enzymes into the cytosolic space.

Results

We demonstrated that these polymeric particles enabled the controlled del

SUBMITTER: Zhan X 

PROVIDER: S-EPMC4449789 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature