A simple assay for glutathione in whole blood.

ABSTRACT: A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.

SUBMITTER: Hakuna L 

PROVIDER: S-EPMC4449837 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature