Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae.

ABSTRACT: BACKGROUND:Adhesion protein E (PE) of Haemophilus influenzae is a 16 - 18 kDa protein with 160 amino acids which causes adhesion to epithelial cells and acts as a major factor in pathogenesis. OBJECTIVES:In this study, we performed cloning, expression and purification of PE as a candidate antigen for vaccine design upon further study. MATERIALS AND METHODS:At first, the pe gene of NTHi ATCC 49766 strain (483 bp) was amplified by PCR. Then, to sequence the resulted amplicon, it was cloned into TA vector (pTZ57R/T). In the next step, the sequenced gene was sub-cloned in pBAD/gIII A vector and transformed into competent Escherichia coli TOP10. For overexpression, the recombinant bacteria were grown in broth medium containing arabinose and the recombinant protein was purified using metal affin

SUBMITTER: Tavakoli M 

PROVIDER: S-EPMC4449846 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature