Isolation and screening of lipolytic fungi from coastal waters of the southern caspian sea (north of iran).

ABSTRACT:

Background

Lipases (acylglycerol acylhydrolase, E. C. 3. 1. 1. 3) are widely distributed among microorganisms, animals and plants, catalyzing the hydrolysis of triglycerides to free fatty acids and glycerol. Their commercial application includes pharmaceutical, chemical, and paper industries.

Objectives

This study aimed to isolate and screen lipolytic fungi from coastal waters of the southern Caspian Sea by Internal Transcribed Spacer-Polymerase Chain Reaction (ITS-PCR), and to optimize their lipolytic activity, pH and temperature. The ITS regions possess a high variation among taxonomically distinct fungal species and even within species.

Materials and methods

All fungal were tested to determine their lipolytic activity by the Tributyrin agar plate assay. After DNA e

SUBMITTER: Sadati R 

PROVIDER: S-EPMC4449855 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature