Progesterone-mediated effects on gene expression and oocyte-cumulus complex transport in the mouse fallopian tube.

ABSTRACT:

Background

The fallopian tube transports the gametes to the fertilization site and delivers the embryo to the uterus at the optimal time for implantation. Progesterone and the classical progesterone receptor are involved in regulating both tubal ciliary beating and muscular contractions, likely via both genomic and non-genomic actions.

Methods

To provide more details of the underlying mechanisms, we investigated the effect of progesterone on gene expression in mice fallopian tubes in vitro at 20 min, 2 h and 8 h post progesterone treatment using microarray and/or quantitative PCR. In parallel, oocyte cumulus complex transport was investigated in ovulating mice that were injected with one of the progesterone receptor antagonists, Org 31710 or CDB2194.

Results

Microarra

SUBMITTER: Bylander A 

PROVIDER: S-EPMC4450483 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature