Creatine kinase B is necessary to limit myoblast fusion during myogenesis.

ABSTRACT: Myoblast fusion is critical for proper muscle growth and regeneration. During myoblast fusion, the localization of some molecules is spatially restricted; however, the exact reason for such localization is unknown. Creatine kinase B (CKB), which replenishes local ATP pools, localizes near the ends of cultured primary mouse myotubes. To gain insights into the function of CKB, we performed a yeast two-hybrid screen to identify CKB-interacting proteins. We identified molecules with a broad diversity of roles, including actin polymerization, intracellular protein trafficking, and alternative splicing, as well as sarcomeric components. In-depth studies of α-skeletal actin and α-cardiac actin, two predominant muscle actin isoforms, demonstrated their biochemical interaction and partial colocaliz

SUBMITTER: Simionescu-Bankston A 

PROVIDER: S-EPMC4451350 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature