A Possible Role for WNT5A Hypermethylation in Pediatric Acute Lymphoblastic Leukemia.

ABSTRACT:

Objective

WNT5A is one of the most studied noncanonical WNT ligands and is shown to be deregulated in different tumor types. Our aim was to clarify whether hypermethylation might be the cause of low WNT5A mRNA levels and whether we could restore this downregulation by reversing the event.

Materials and methods

The expression of WNT5A mRNA was studied in a large acute lymphoblastic leukemia (ALL) patient group (n=86) by quantitative real-time PCR. The methylation status was detected by methylation-specific PCR (MSPCR) and bisulphate sequencing. In order to determine whether methylation has a direct effect on WNT5A expression, disease-representative cell lines were treated by 5'-aza-20-deoxycytidine.

Results

Here we designed a validation experiment of the WNT5A gene, wh

SUBMITTER: Hatırnaz Ng O 

PROVIDER: S-EPMC4451480 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature