Development of a non-infectious encapsidated positive control RNA for molecular assays to detect foot-and-mouth disease virus.

ABSTRACT: Positive controls are an important component of the quality-control of molecular tests used for diagnosis of livestock diseases. For high consequence agents such as foot-and-mouth disease virus (FMDV), the positive controls required to monitor template extraction, reverse transcription and amplification steps usually consist of material derived from infectious viruses. Therefore, their production is dependent upon the use of high containment facilities and their deployment carries the risks associated with inactivation of "live" FMDV. This paper describes the development of a novel non-infectious positive control that encodes FMDV RNA sequences that are encapsidated within Cowpea mosaic virus (CPMV) particles. This surrogate RNA has been engineered to contain sequences from the 5'UTR and 3

SUBMITTER: Madi M 

PROVIDER: S-EPMC4451496 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature