Crystal structure of the fibre head domain of bovine adenovirus 4, a ruminant atadenovirus.

ABSTRACT:

Background

In adenoviruses, primary host cell recognition is generally performed by the head domains of their homo-trimeric fibre proteins. This first interaction is reversible. A secondary, irreversible interaction subsequently takes place via other adenovirus capsid proteins and leads to a productive infection. Although many fibre head structures are known for human mastadenoviruses, not many animal adenovirus fibre head structures have been determined, especially not from those belonging to adenovirus genera other than Mastadenovirus.

Methods

We constructed an expression vector for the fibre head domain from a ruminant atadenovirus, bovine adenovirus 4 (BAdV-4), consisting of amino acids 414-535, expressed the protein in Escherichia coli, purified it by metal affinity and

SUBMITTER: Nguyen TH 

PROVIDER: S-EPMC4451742 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature