Automated detection of whole-cell mitochondrial motility and its dependence on cytoarchitectural integrity.

ABSTRACT: Current methodologies used for mitochondrial motility analysis tend to either overlook individual mitochondrial tracks or analyze only peripheral mitochondria instead of mitochondria in all regions of the cell. Furthermore, motility analysis of an individual mitochondrion is usually quantified by establishing an arbitrary threshold for "directed" motion. In this work, we created a custom, publicly available computational algorithm based on a previously published approach (Giedt et al., 2012. Ann Biomed Eng 40:1903-1916) in order to characterize the distribution of mitochondrial movements at the whole-cell level, while still preserving information about single mitochondria. Our technique is easy to use, robust, and computationally inexpensive. Images are first pre-processed for increased re

SUBMITTER: Kandel J 

PROVIDER: S-EPMC4452426 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature